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ASMA Training _ Training Program

  • Introduction
  • Training Program
  • Regular Validation Follow-Up
  • Trainees photos and country
  • How to participate: Application forms etc

Asia Standardization of Molecular Assay (ASMA) Training

Main Projects - ASMA Training

The training provides various molecular assays used in diagnosis and treatment of chronic myeloid leukemia.
The molecular biology technologies involved in molecular assays are as follow:

  • - Real-time quantitative PCR (RQ-PCR): to measure BCR-ABL transcript level
  • - Multiplex PCR: to identify the type of BCR-ABL transcripts
  • - Direct sequencing & Allele specific oligonucleotide PCR (ASO-PCR): to detect mutations in Bcr-Abl kinase
    domain
  • - Molecular cloning: to prepare reference and target plasmid standards for absolute quantification in RQ-PCR.
  • - Other technologies: White blood cell isolation, RNA extraction & quality check, DNA extraction, reverse
    transcription, gel electrophoresis etc.
  • - Quantitation of imatinib in human plasma

    • According to training duration, three different courses will be provided.

  • - Yellow course (1 week) covers RQ-PCR
  • - Blue course (2 weeks) covers RQ-PCR and mutation analysis
  • - Red course (3 weeks) covers RQ-PCR, mutation analysis and molecular cloning
  • - Black coure (4 weeks) covers RQ-PCR, mutation analysis, molecular cloning and pharmacokinetics.
    • Training Program
    Assay Technology Yellow
    1 week    		 Blue
    2 weeks    		 Red
    3 weeks    		 Black
    4 weeks
    BCR-ABL
    transcript type
    &
    transcript level
    analysis    		 White blood cell purification O O O O
    RNA isolation & quality check O O O O
    First strand cDNA synthesis O O O O
    Multiplex PCR O O O O
    RQ-PCR O O O O
    Real time PCR data analysis O O O O
    Mutation analysis in
    Bcr-Abl
    kinase domain    		 Long template PCR   O O O
    Nested PCR   O O O
    Big Dye termination PCR   O O O
    DNA sequencing   O O O
    DNA sequencing data analysis   O O O
    DNA extraction & ASO-PCR   O O O
    Molecular cloning Plasmid ligation     O O
    Transformation of competent bacteria     O O
    Colony formation     O O
    Colony PCR     O O
    Recombinant plasmid preparation     O O
    Pharmaco-kinetics HPLC       O
    Tandem Mass Spectrometry       O

    Training for each assay consists of the following components;

  • - Introduction of background knowledge and technical information by training staff
  • - Full demonstration of every step of each assay by qualified demonstrator.
  • - Practical performance of the demonstrated steps by trainee under the supervision of demonstrator.
  • - Actual performance of whole assay by trainee

    • Yellow Course (1 week)

    Training Yellow Program (week1)
    Week 1 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Molecular Assay Training    		 Purification of
    white blood cells
    from peripheral blood
    (by Trainee)    		 RQ-PCR
    (by Demonstrator)    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Introduction of
    Asia CML Registry
    RQ-PCR optimization
    (by Demonstrator)
    Analysis of
    RQ-PCR data
    (by Demonstrator)
    Purification of
    white blood cells
    from peripheral blood
    (by Demonstrator)    		 RNA extraction
    (by Trainee)    		 cDNA synthesis
    (by Trainee)    		 Asia CML Registry
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ RNA extraction
    (by Demonstrator)
    		 RNA quality check
    (by Trainee)    		 RQ-PCR
    (by Trainee)
    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Summary of Molecular
    Assay training
    cDNA synthesis
    (by Demonstrator)    		 Multiplex PCR
    (by Demonstrator)
    RNA quality check
    (by Demonstrator)    		 Introduction of
    RQ-PCR    		 Analysis of
    RQ-PCR data
    (by Trainee)    		 Question & Answer
    (by All)

    Blue Course (2 weeks)

    Training Blue Program (week1)
    Week 1 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Molecular Assay Training    		 Purification of
    white blood cells
    from peripheral blood
    (by Trainee)    		 RQ-PCR
    (by Demonstrator)    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Introduction of
    Asia CML Registry
    RQ-PCR optimization
    (by Demonstrator)
    Analysis of
    RQ-PCR data
    (by Demonstrator)
    Purification of
    white blood cells
    from peripheral blood
    (by Demonstrator)    		 RNA extraction
    (by Trainee)    		 cDNA synthesis
    (by Trainee)    		 Asia CML Registry
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ RNA extraction
    (by Demonstrator)
    		 RNA quality check
    (by Trainee)    		 RQ-PCR
    (by Trainee)
    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Summary of Molecular
    Assay training
    cDNA synthesis
    (by Demonstrator)    		 Multiplex PCR
    (by Demonstrator)
    RNA quality check
    (by Demonstrator)    		 Introduction of
    RQ-PCR    		 Analysis of
    RQ-PCR data
    (by Trainee)    		 Question & Answer
    (by All)
    Training Blue Program (week2)
    Week 2 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Mutation Analysis
    		 Clean up of sequencing
    PCR
    (by Demonstrator)    		 Clean up
    of sequencing PCR
    (by Trainee)
    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Introduction of
    ASO-PCR
    1st PCR
    (by Demonstrator)
    		 Sequencing
    (by Demonstrator)
    		 Sequencing
    (by Trainee)    		 ASO-PCR
    (by Demonstrator)
    1st PCR
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ 2nd PCR
    (by Demonstrator)
    		 2nd PCR
    (by Trainee)
    Sequencing data
    analysis
    (by Demonstrator)    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Patient Review
    &
    How to apply lab
    results into
    clinical paper
    PCR Gel loading
    (by Trainee)
    Introduction of
    Big Dye
    termination sequencing    		 2nd PCR purification
    (by Trainee)    		 Sequencing data
    analysis
    (by Trainee)
    		 Summary of Molecular
    Assay training
    Question & Answer
    (by All)
    PCR Gel loading &
    PCR purification
    (by Demonstrator)
    Sequencing (Big Dye)
    PCR
    (by Demonstrator)    		 Sequencing (Big Dye)
    PCR (by Trainee)

    Red Course (3 weeks)

    Training Red Program (week1)
    Week 1 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Molecular Assay Training    		 Purification of
    white blood cells
    from peripheral blood
    (by Trainee)    		 RQ-PCR
    (by Demonstrator)    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Introduction of
    Asia CML Registry
    RQ-PCR optimization
    (by Demonstrator)
    Analysis of
    RQ-PCR data
    (by Demonstrator)
    Purification of
    white blood cells
    from peripheral blood
    (by Demonstrator)    		 RNA extraction
    (by Trainee)    		 cDNA synthesis
    (by Trainee)    		 Asia CML Registry
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ RNA extraction
    (by Demonstrator)
    		 RNA quality check
    (by Trainee)    		 RQ-PCR
    (by Trainee)
    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Summary of Molecular
    Assay training
    cDNA synthesis
    (by Demonstrator)    		 Multiplex PCR
    (by Demonstrator)
    RNA quality check
    (by Demonstrator)    		 Introduction of
    RQ-PCR    		 Analysis of
    RQ-PCR data
    (by Trainee)    		 Question & Answer
    (by All)
    Training Red Program (week2)
    Week 2 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Mutation Analysis
    		 Clean up of sequencing
    PCR
    (by Demonstrator)    		 Clean up
    of sequencing PCR
    (by Trainee)
    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Introduction of
    ASO-PCR
    1st PCR
    (by Demonstrator)
    		 Sequencing
    (by Demonstrator)
    		 Sequencing
    (by Trainee)    		 ASO-PCR
    (by Demonstrator)
    1st PCR
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ 2nd PCR
    (by Demonstrator)
    		 2nd PCR
    (by Trainee)
    Sequencing data
    analysis
    (by Demonstrator)    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Patient Review
    &
    How to apply lab
    results into
    clinical paper
    PCR Gel loading
    (by Trainee)
    Introduction of
    Big Dye
    termination sequencing    		 2nd PCR purification
    (by Trainee)    		 Sequencing data
    analysis
    (by Trainee)
    		 Summary of Molecular
    Assay training
    Question & Answer
    (by All)
    PCR Gel loading &
    PCR purification
    (by Demonstrator)
    Sequencing (Big Dye)
    PCR
    (by Demonstrator)    		 Sequencing (Big Dye)
    PCR (by Trainee)
    Training Red Program (week3)
    Week 3 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of cloning Colony selection
    (by Demonstrator)    		 Introduction of plasmid preparation
    		 Introduction of standard in RQ-PCR Colony selection
    (by Trainee)
    Test of the prepared plasmid as standard in RQ-PCR
    (by Demonstrator)
    Preparation of insert (PCR & clean-up of PCR product)
    (by Demonstrator)
    		 Colony PCR for selecting positive clone
    (by Demonstrator)
    		 Mini-preparation of recombinant plasmid
    (by Demonstrator)
    		 Preparation of insert by PCR (by Trainee) Colony PCR for selecting positive clone
    &
    Gel electrophoresis
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ Ligation with cleaned PCR product and plasmid
    (by Demonstrator)
    		 Gel electrophoresis
    (by Demonstrator)
    Mini-preparation of recombinant plasmid
    (by Demonstrator)

    Clean-up of PCR product & Ligation
    (by Trainee)

    		 Summary of Molecular Assay training
    Transforamtion of competent cells
    (by Demonstrator)
    		 Preparation of LB broth
    (by Demonstrator)
    		 Check of insert through PCR and restricted endonuclease digestion
    (by Demonstrator)    		 Transforamtion of competent cells
    (by Trainee)    		 Question & Answer
    (by All)
    Preparation of LB plate & Plating
    (by Demonstrator)
    Mini-culture of selected colonies
    (by Demonstrator)    		 Preparation of LB plate & Plating
    (by Trainee)

    Black Course (4 weeks)

    Training Black Program (week1)
    Week 1 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Molecular Assay Training    		 Purification of
    white blood cells
    from peripheral blood
    (by Trainee)    		 RQ-PCR
    (by Demonstrator)    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Introduction of
    Asia CML Registry
    RQ-PCR optimization
    (by Demonstrator)
    Analysis of
    RQ-PCR data
    (by Demonstrator)
    Purification of
    white blood cells
    from peripheral blood
    (by Demonstrator)    		 RNA extraction
    (by Trainee)    		 cDNA synthesis
    (by Trainee)    		 Asia CML Registry
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ RNA extraction
    (by Demonstrator)
    		 RNA quality check
    (by Trainee)    		 RQ-PCR
    (by Trainee)
    		 Practice Test:

    From Purification of
    white blood cells
    to RQ-PCR
    (by Trainee)

    		 Summary of Molecular
    Assay training
    cDNA synthesis
    (by Demonstrator)    		 Multiplex PCR
    (by Demonstrator)
    RNA quality check
    (by Demonstrator)    		 Introduction of
    RQ-PCR    		 Analysis of
    RQ-PCR data
    (by Trainee)    		 Question & Answer
    (by All)
    Training Black Program (week2)
    Week 2 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of
    Mutation Analysis
    		 Clean up of sequencing
    PCR
    (by Demonstrator)    		 Clean up
    of sequencing PCR
    (by Trainee)
    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Introduction of
    ASO-PCR
    1st PCR
    (by Demonstrator)
    		 Sequencing
    (by Demonstrator)
    		 Sequencing
    (by Trainee)    		 ASO-PCR
    (by Demonstrator)
    1st PCR
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ 2nd PCR
    (by Demonstrator)
    		 2nd PCR
    (by Trainee)
    Sequencing data
    analysis
    (by Demonstrator)    		 Practice Test:

    From 1st PCR to
    sequencing
    (by Trainee)

    		 Patient Review
    &
    How to apply lab
    results into
    clinical paper
    PCR Gel loading
    (by Trainee)
    Introduction of
    Big Dye
    termination sequencing    		 2nd PCR purification
    (by Trainee)    		 Sequencing data
    analysis
    (by Trainee)
    		 Summary of Molecular
    Assay training
    Question & Answer
    (by All)
    PCR Gel loading &
    PCR purification
    (by Demonstrator)
    Sequencing (Big Dye)
    PCR
    (by Demonstrator)    		 Sequencing (Big Dye)
    PCR (by Trainee)
    Training Black Program (week3)
    Week 3 Day 1 Day 2 Day 3 Day 4 Day 5
    AM 9:00 ~ Introduction of cloning Colony selection
    (by Demonstrator)    		 Introduction of plasmid preparation
    		 Introduction of standard in RQ-PCR Colony selection
    (by Trainee)
    Test of the prepared plasmid as standard in RQ-PCR
    (by Demonstrator)
    Preparation of insert (PCR & clean-up of PCR product)
    (by Demonstrator)
    		 Colony PCR for selecting positive clone
    (by Demonstrator)
    		 Mini-preparation of recombinant plasmid
    (by Demonstrator)
    		 Preparation of insert by PCR (by Trainee) Colony PCR for selecting positive clone
    &
    Gel electrophoresis
    (by Trainee)
    PM12:00 ~ Lunch Lunch Lunch Lunch Lunch
    PM 1:30 ~ Ligation with cleaned PCR product and plasmid
    (by Demonstrator)
    		 Gel electrophoresis
    (by Demonstrator)
    Mini-preparation of recombinant plasmid
    (by Demonstrator)

    Clean-up of PCR product & Ligation
    (by Trainee)

    		 Summary of Molecular Assay training
    Transforamtion of competent cells
    (by Demonstrator)
    		 Preparation of LB broth
    (by Demonstrator)
    		 Check of insert through PCR and restricted endonuclease digestion
    (by Demonstrator)    		 Transforamtion of competent cells
    (by Trainee)    		 Question & Answer
    (by All)
    Preparation of LB plate & Plating
    (by Demonstrator)
    Mini-culture of selected colonies
    (by Demonstrator)    		 Preparation of LB plate & Plating
    (by Trainee)
    Training Black Program (week4)
    Week 4 Day 1 Day 2 Day 3 Day 4
    AM 9:00 ~ Introduction to
    bioanalytical method validation
    &
    HPLC/MS spectrometry
    		 Extraction methods Acceptance criteria in validation
    		 Acceptance criteria in sample analysis
    Quality control preparation
    (by Demonstrator)    		 Unknown sample extraction
    &
    HPLC/MS operation
    (by Trainee)
    		 Total review of Training
    PM12:00 ~ Lunch Lunch Lunch Lunch
    PM 1:30 ~ Stock and mobile phase preparation
    &
    HPLC/MS operation
    (by Demonstrator)    		 Quality control extraction
    &
    HPLC/MS operation
    (by Demonstrator)    		 Continued

    Question & Answer
    (by All)
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