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ASMA Training _ Regular Validation Follow-Up

  • Introduction
  • Training Program
  • Regular Validation Follow-Up
  • Trainees photos and country
  • How to participate: Application forms etc

ASMA is aiming to provide the qualified data for ACR

  • • Standardized molecular analysis
  • • Future collaborating clinical studies
  • Cross validation for Molecular Assays for RQ-PCR and mutation analysis

  • • Creating lab-specific conversion factors using sample exchange
  • • Measuring BCR-ABL transcript levels in IS
  • • Measuring amount of mutant clone
  • • Validation of BCR-ABL mutations using qualified DS
  • Samples

  • RQ-PCR
  • - CML cell line (K562)
  • - Serial dilution with normal cell lines (HL60 or KG-1)
  • - At High level of BCR-ABL
  • - At low level of BCR-ABL
  • Mutation
  • - CML cell line (K562)
  • - Serial dilution with normal cell lines (HL60 or KG-1)
  • - At High level of BCR-ABL
  • - At low level of BCR-ABL
  • Regardless of RQ-PCR machines (Roche, ABI etc…)
  • Regardless of DNA sequencing machines

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